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Primary Cells

Human Frozen/Untouched PB CD19+/CD27+ Memory B cells

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NPB-CD19+/CD27+ memory B cells are negatively isolated from Mononuclear Cells using immunomagnetic isolation system to deplete non-memory B cells.
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  • High Purity Levels
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Species

Human

Cat.No

ABC-TC3438

Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Product Category Primary Cells
Size/Quantity

1 vial

Cell Type

B Cell

Shipping Info

Dry Ice

Growth Conditions

37 ℃, 5% CO2

Source Organ

Peripheral Blood

Disease

Normal

Biosafety Level

1

Storage

Liquid Nitrogen

Product Type

Immune Cells

Description

Human Frozen/Untouched PB CD19+/CD27+ Memory B cellsHuman Frozen/Untouched PB CD19+/CD27+ Memory B cells are primary B lymphocytes isolated from human peripheral blood mononuclear cells (PBMCs). These cells are enriched through two-step immunomagnetic separation: non-memory B cells are depleted to yield a CD19+ fraction, followed by positive selection with anti-CD27 microbeads. They exhibit high purity and viability, verified by flow cytometry prior to cryopreservation. Morphologically, they are non-adherent immune cells that remain dormant after antigen exposure, enabling rapid antibody response upon re-encounter. Functionally, they are critical for studying immunological memory and applications include evaluating therapeutic antibodies, autoimmune disease treatments, and viral infection response. Cells are collected under IRB-approved protocols with informed consent and screened for HIV-1, HIV-2, HBV, and HCV.

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Citation

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Application

  • Human Frozen/Untouched PB CD19+/CD27+ Memory B cells can be used in multiple research areas. They act as a key system to test antibody-based therapies, enabling the assessment of maintenance regimens for disorders like neuromyelitis optica spectrum disorder. This evaluation supports the development of personalized treatment approaches, and help minimize dose while preserving effectiveness. Additionally, these cells contribute to understanding the immune response to viral infections, enabling the development of more effective vaccines and antiviral strategies. Contrasting them with other B cell types provides insights into unique immunological traits and mechanisms underlying long-term immunity, aiding in the optimization of targeted therapeutic interventions.

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High Viability
To succeed in cell culture
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To support a consistent result
Customization Options
Tailed to your research

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