Human Lobar Bronchial Epithelial Cells

Description

Human Lobar Bronchial Epithelial Cells (HLBECs) are primary cells derived from the lobar bronchi. These cells are classified as pseudostratified columnar epithelial cells, characterized by their tall, columnar shape and nuclei positioned at varying heights, giving the appearance of stratification despite being a single cell layer. These bronchial cells exhibit apical cilia and microvilli, as well as secretory granules in goblet cells, which are interspersed among the ciliated cells. HLBECs play critical roles in mucociliary clearance, airway defense via mucus secretion, and barrier function against inhaled pathogens and pollutants. Key molecular markers expressed in HLBECs include epithelial-associated markers such as cytokeratin 18 (CK18), E-cadherin, and zonula occludens-1 (ZO-1), which are commonly used to confirm epithelial cell identity and cell–cell junction integrity. To ensure the quality of these primary cells, repeated freeze-thaw cycles are not recommended due to the fragile nature of these cells. The cells are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, syphilis, mycoplasma, fungi, yeast, and bacteria.