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Human NRAS (G12C_A59T) BAF3 Cell Line

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Home | Transfected Stable Cell Lines | Overexpression Stable Cell Lines | Human NRAS (G12C_A59T) BAF3 Cell Line
Species

Human
Cat.No

ABC-X0281C
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Product Category Transfected Stable Cell Lines
Size/Quantity

1 vial
Cell Type

Lymphocytes
Shipping Info

Dry Ice
Growth Conditions

37 °C, 5% CO2
Source Organ

Lymphatic
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Overexpression Stable Cell Lines
Host Cell

BAF3

Description

Human NRAS (G12C_A59T) BAF3 Cell Line is a genetically engineered model derived from murine pro-B Ba/F3 parental cells based on customers’ requirement. NRAS (G12C_A59T) BAF3 overexpression cell line is generated by stable integration of exogenous human NRAS carrying G12C and A59T double mutations into Ba/F3 host cells using our optimized lentiviral vector transduction system. Overexpression clone is validated at gene level by qRT-PCR.
Target
NRAS is a member of the RAS oncogene family involved in signaling pathways such as MAPK and PI3K/AKT. The G12C and A59T mutations cooperate to enhance oncogenic potential through altered GTPase activity and signaling output. These variants are linked to hematologic malignancies and RASopathy syndromes. AcceGen offers generation of stable overexpression cell lines targeting any gene of your interest. Polyclonal or monoclonal is optional based on customers’ research needs.

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Application

  • For research use only

  • This model is suitable for investigating dual mutation synergy in NRAS signaling, drug resistance mechanisms, and testing mutation-specific inhibitors or pathway blockers.

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