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Transfected Stable Cell Lines

Human NRAS (G12D_Y96D) BAF3 Cell Line

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Home | Transfected Stable Cell Lines | Overexpression Stable Cell Lines | Human NRAS (G12D_Y96D) BAF3 Cell Line
Species

Human
Cat.No

ABC-X0283C
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Product Category Transfected Stable Cell Lines
Size/Quantity

1 vial
Cell Type

Lymphocytes
Shipping Info

Dry Ice
Growth Conditions

37 °C, 5% CO2
Source Organ

Lymphatic
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Overexpression Stable Cell Lines
Host Cell

BAF3

Description

Human NRAS (G12D_Y96D) BAF3 Cell Line is a genetically engineered model derived from murine Ba/F3 cells via stable integration of human NRAS gene with G12D and Y96D point mutations. Lentiviral delivery ensures sustained expression, and edited cells are expanded at low passage (<P20). Validation is performed at gene level using qRT-PCR. Target NRAS mutations at G12D are frequently observed in acute myeloid leukemia and melanoma, promoting constitutive MAPK activation. The additional Y96D mutation modulates effector binding and downstream signaling. AcceGen offers generation of stable overexpression cell lines targeting any gene of your interest. Polyclonal or monoclonal is optional based on customers' research needs.

AcceGen Frozen Cells & Cell Lines 1 vial
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Application

  • For research use only

  • This dual-mutant NRAS BAF3 line supports research on NRAS-driven transformation, therapeutic resistance pathways, and screening of selective inhibitors in hematological and skin cancers.

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