Human Osteoblasts, Fetal

Description

Human Osteoblasts, Foetal are primary bone-forming cells isolated from healthy fetal calvarial or long bone tissue. These adherent, mesenchymal-derived cells exhibit characteristic cuboidal morphology and robust alkaline phosphatase (ALP) activity, essential for early matrix deposition and mineralization. They constitutively express key osteogenic markers including COL1A1, secreted osteocalcin (BGLAP), and osteopontin (SPP1), as well as angiogenesis-modulating receptors including VEGFR1 (FLT1), VEGFR2 (KDR), and VEGFR3 (FLT4), which regulate angiogenesis and osteogenesis during fetal bone development. After isolation, cells are cryopreserved at early passages to conserve phenotype. As non-immortalized primary cells, they have finite proliferative capacity and are not suitable for long-term passaging. Each lot undergoes rigorous screening and isolation procedures, and is rigorously tested to ensure it is free of contamination from HIV-1, HBV, HCV, Syphilis, Mycoplasma, Fungi, Yeast, and Bacteria.