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Human PIK3CA (G1049R) BAF3 Cell Line

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Home | Transfected Stable Cell Lines | Overexpression Stable Cell Lines | Human PIK3CA (G1049R) BAF3 Cell Line
Species

Human
Cat.No

ABC-X0285C
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Product Category Transfected Stable Cell Lines
Size/Quantity

1 vial
Cell Type

Lymphocytes
Shipping Info

Dry Ice
Growth Conditions

37 °C, 5% CO2
Source Organ

Lymphatic
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Overexpression Stable Cell Lines
Host Cell

BAF3

Description

Human PIK3CA (G1049R) BAF3 Cell Line is a genetically engineered model derived from murine pro-B Ba/F3 cells with stable overexpression of human PIK3CA harboring the G1049R mutation. Lentiviral transduction was employed to generate this model, which is maintained at low passage numbers (<P20). Overexpression is confirmed by qRT-PCR. Target G1049R is a gain-of-function mutation located within the kinase domain of PIK3CA, enhancing catalytic activity and PI3K/AKT signaling. It is identified in multiple tumor types including breast and ovarian cancers. AcceGen offers generation of stable overexpression cell lines targeting any gene of your interest. Polyclonal or monoclonal is optional based on customers' research needs.

AcceGen Frozen Cells & Cell Lines 1 vial
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Application

  • For research use only

  • This cell line provides a robust system for characterizing PIK3CA mutant oncogenic signaling, evaluating PI3K inhibitors, and understanding mechanisms of pathway reactivation in resistant tumor models.

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