Human Prostate Fibroblasts

Description

Human prostate fibroblasts (HPF) are primary stromal cells derived from non-malignant prostate tissue, exhibiting characteristic bipolar, spindle-shaped morphology with robust adherence in culture. They play a crucial role in prostate physiology by producing extracellular matrix (ECM) proteins such as collagen I/III and fibronectin, and secretion of growth factors like bFGF, TGF β, IGFs, and KGF that mediate epithelial–stromal interactions. Immunophenotypically, they typically express vimentin while remaining α-SMA negative unless activated. In prostate cancer research, fibroblasts from different zones can enhance tumor progression and castration resistance through paracrine effects in co culture models. These cells have a limited proliferative capacity and are unsuitable for indefinite passaging. Each lot undergoes rigorous screening and isolation procedures, and is rigorously tested to ensure it is free of contamination from HIV-1, HBV, HCV, Syphilis, Mycoplasma, Fungi, Yeast, and Bacteria.