Human Stomach Cancer Peripheral Blood Mononuclear Cells

Description

Human Stomach Cancer Peripheral Blood Mononuclear Cells (PBMCs) are isolated from peripheral blood of patients with histologically confirmed gastric adenocarcinoma, including both intestinal and diffuse subtypes. These human-derived PBMCs contain immune cell populations (T cells, B cells, NK cells, monocytes) that exhibit gastric cancer-specific immune alterations, including tumor-driven immunosuppression, marked by Tregs expansion (FoxP3⁺), MDSC infiltration (CD14⁺HLA-DR⁻/low), exhausted CD8⁺ T cells (PD-1⁺TIM-3⁺), and Th17/IL-17 dominance, promoting immune evasion and metastasis. Monocytes differentiate into tumor-associated macrophages (TAMs) facilitate angiogenesis and metastasis through VEGF and MMP secretion. The cells display typical mononuclear morphology with intact surface markers and maintain functional cytokine production (IL-10, TGF-β, IFN-γ) characteristic of the immunosuppressive gastric tumor microenvironment. They are particularly valuable for studying the distinct immunobiology of EBV-positive versus microsatellite instability-high (MSI-H) gastric cancers, which show differential responses to immunotherapy. These PBMCs offer a valuable model to investigate the tumor microenvironment PBMC, identify potential therapeutic targets gastric cancer, support detailed PBMC isolation stomach cancer, characterize stomach cancer immune cells, and profile functional cytokines PBMC gastric cancer.