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Primary Cells

Human T Cells-Negatively Selected

  • For research use only

Cat No.

ABC-TC3830
Product Type

Immune Cells
Cell Type

T Cell
Species

Human
Growth Conditions

37 ℃, 5% CO2
Source Organ

Peripheral Blood
Disease

Normal
Storage

Liquid Nitrogen

Human T Cells-Negatively Selected(HTC) are purified from healthy donors and ready to use in studies of T cell biology.

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  • Human T Cells-Negatively Selected
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Home | Primary Cells | Human Primary Cells | Immune System Primary Cells | Human T Cells-Negatively Selected

Description

Human T Cells-Negatively Selected (hT-NS) are isolated from peripheral blood mononuclear cells (PBMCs) collected from healthy human donors using density gradient centrifugation followed by negative selection. This method removes non-T cell populations (e.g., B cells, NK cells, monocytes, dendritic cells, leaving a highly enriched population of CD3+ T cells). hT-NS display a small, round morphology and are primarily composed of both CD4+ helper and CD8⁺ cytotoxic subsets. They express surface markers such as CD3, CD4, CD8, and TCRαβ. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, Mycoplasma, Fungi, Yeast, and Bacteria.
Product Code

Human T Cells-Negatively Selected, Untouched T Cells, Pan T Cells (Negative Selection), Enriched T Lymphocytes
Species

Human
Cat.No

ABC-TC3830
Product Category

Primary Cells
Size/Quantity

1 vial
Cell Type

T Cell
Growth Mode

Suspension
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Peripheral Blood
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Immune Cells
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Application

  • Human negatively selected T cells (hT-NS) provide a critical ex vivo model for immunological research while maintaining native receptor expression and an unstimulated functional state. These untouched lymphocytes are suitable for investigating physiological T cell activation thresholds, antigen-specific responses, immune checkpoint interactions, and TCR signaling dynamics. Their unaltered state enables accurate assessment of cytotoxic function and immune activation in primary cell systems. hT-NS also serve as starting material for CAR-T cell engineering, supporting studies of T cell activation, expansion, and viral transduction efficiency.

Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

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