Immortalized Human Adipose-Derived Mesenchymal Stem Cells

Frequently Asked Questions

• Can these cells be passaged and cryopreserved in liquid nitrogen?

  Yes. The cells can be routinely passaged and cryopreserved in liquid nitrogen for long-term storage.

• How many passages are possible?

  The cells can be passaged at least 10 times or more under recommended culture conditions.

• What culture medium is required for growth and differentiation?

  For routine culture, use Mesenchymal Stem Cell Medium, composed of:

  500 mL Basal Medium

  25 mL FBS

  5 mL Mesenchymal Stem Cell Growth Supplement

  5 mL Penicillin/Streptomycin (P/S) solution

  For differentiation experiments, please refer to the corresponding differentiation protocol or relevant literature, as specific differentiation media may vary depending on the target lineage.