Ishikawa

The Ishikawa cell line is a human endometrial adenocarcinoma cell line established from a 39-year-old woman. These epithelial-like cells exhibit a mosaic monolayer growth pattern and tend to pile, mirroring the structure of the original tumor. They demonstrate tumorigenicity in athymic nude mice, forming well-differentiated adenocarcinomas upon transplantation. Ishikawa cells express estrogen and progesterone receptors both in vitro and in vivo, making them an ideal in vitro model for studying estrogenic responses. Genetically, they display a diploid chromosomal range with recurrent translocation t(9;14), and harbor mutations in TP53 and POLE, while being microsatellite instability-high (MSI-H) with low copy number variation. Additionally, these cells secrete hormones such as corticotropin-releasing hormone, placental alkaline phosphatase, and chorionic gonadotrophin, and express integrins including α2β1, α3β1, and α6β4.