KNS-42

Description

The KNS-42 cell line is a human glioma cell line derived from a malignant glioma in a 16-year-old Japanese male patient, classified as grade IV glioblastoma. These cells exhibit tumor angiogenesis in vitro and can form tumors when transplanted into immunodeficient mice. In sparse culture, the cells appear polygonal and flat, while in dense culture, they are mainly polygonal with some smaller cells. They carry mutations in the BLM gene (p.Ser186_Lys187delinsTer) and TP53 gene (p.Arg342Ter). The cells express GFAP, NSE, vimentin, fibronectin, and high levels of stem cell markers like CD133, Nestin, SOX2, and Musashi-1. KNS-42 cells are tumorigenic and form tumors in immunodeficient mice, but their metastatic potential has not been fully explored.

Application

• The KNS-42 cell line has various applications in glioma research. It serves as a valuable tool to evaluate the effectiveness of potential therapies, including radiation, for human glioma treatment. Additionally, KNS-42 cells are utilized to study the migratory characteristics of adult glioma cells and evaluate the impact of small molecule inhibitors targeting migration. Furthermore, this cell line is employed in investigating key oncogenes involved in glioblastoma, focusing on their role in epithelial-mesenchymal transition induction, a critical process in tumor progression.