For research use only
| Cat No. | ABC-BR0015 |
Lymphocytes Subgroup Typing Kit is primarily used to determine the percentage of various peripheral blood lymphocyte subsets relative to total lymphocytes.
Human peripheral blood lymphocytes can be divided into three groups based on their biological functions and cell surface antigen expression: T lymphocytes, B lymphocytes, and NK cells. T lymphocytes primarily participate in cellular immune responses mediated by specific antigens and regulate immunoglobulin secretion by B lymphocytes. T lymphocytes can be further divided into helper/inducer T cells and suppressor/cytotoxic T cells based on their functions. NK cells, or natural killer cells, mediate cytotoxicity against certain tumor cells and virus-infected cells, independent of the presence of MHC class I and II molecules.
The Human Lymphocyte Subpopulation Analysis Kit, comprised of a series of dual-color fluorescently labeled antibodies, is primarily used to determine the percentage of various peripheral blood lymphocyte subsets relative to total lymphocytes, including mature T lymphocytes (CD3+), helper/inducer T cells (Th/i: CD3+CD4+), suppressor/cytotoxic T cells (Ts/c: CD3+CD8+), B lymphocytes (CD19+), and NK cells (CD3-CD16+ and/or CD56+). The Th/Ts (CD3+CD4+/CD3+CD8+) cell ratio can also be calculated.
| Cat.No | ABC-BR0015 |
| Product Category | Cell Culture Media, Kits & Reagents |
| Size/Quantity | 50 T |
| Storage | 4℃, protect from light |
Flow cytometry was used with an excitation wavelength of 488 nm and an emission wavelength of 578 nm. The orange-red fluorescence of PE was detected in the FL2 channel. The maximum excitation wavelength of FITC is 488 nm and the maximum emission wavelength is 525 nm. The green fluorescence of FITC was detected in the FL1 channel. Compared to traditional methods for analyzing lymphocyte subsets, flow cytometry combines the specificity of antibodies with the sensitivity of single-cell analysis using flow cytometry, making this analysis more accurate and convenient.