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Species | Mouse |
Cat.No | ABC-HP029X |
Quality Control | All cells test negative for mycoplasma, bacteria, yeast, and fungi. |
Product Category | Primary Cells |
Size/Quantity | 1 vial |
Cell Type | Monocyte |
Shipping Info | Dry Ice |
Growth Conditions | 37 ℃, 5% CO2 |
Disease | Normal |
Storage | Liquid Nitrogen |
Product Type | Mouse Primary Cells |
Mouse Peripheral Blood Mononuclear Cells (PBMCs) are primary cells isolated from the peripheral blood of healthy or disease-model mice, including those with tumors or autoimmune disorders. As non-passagable cells, PBMCs are freshly prepared by density gradient centrifugation and exhibit suspended growth in vitro. They consist of immune subsets such as T cells (CD3⁺), B cells (CD19⁺), and NK cells (CD56⁺), identified via flow cytometry. PBMCs maintain a normal diploid karyotype (2n=40), confirmed by G-banding. Functionally, they participate in both innate and adaptive immunity, with cytokine production and immune modulation assessed by ELISA and cytotoxicity assays. These cells are widely used in studies on tumor immunity, autoimmune diseases, and infectious models.
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Mouse PBMCs are widely used in immunology research. They are commonly employed in in-vitro immunoassays, including ELISPOT, mixed lymphocyte reactions, cytotoxicity assays to evaluate antibody-dependent cell-mediated cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC), and plaque assays. They are also used in ex-vivo studies to characterize cell populations and create genetically modified cells using retroviral infection techniques. These versatile cells are essential in many immunology applications, such as drug discovery and development, assay validation and development, and other research areas that require a reliable model for investigating immune responses and evaluating therapeutic interventions.