MST4 Knockout 293T Cell Line

Description

MST4 Knockout 293T Cell Line is generated from human embryonic kidney 293T cells through CRISPR/Cas9-mediated disruption of the Mammalian Sterile 20-like kinase 4 (MST4/STK26) gene, a key regulator of cell polarity, Golgi apparatus organization, and inflammatory responses. This knockout model exhibits impaired phosphorylation of downstream substrates including STK25 while maintaining 293T cells’ characteristic adherent growth with epithelial morphology and high transfection efficiency. The cell line serves as an essential tool for studying cell migration, immunological synapse formation, and tumor metastasis, particularly in research involving ERM (ezrin-radixin-moesin) protein regulation and TLR4-mediated NFκB activation. Maintained at low passage numbers (<P20) with genetic stability, the knockout efficiency is validated through genomic PCR, Sanger sequencing, and phospho-proteomic analysis demonstrating loss of MST4 kinase activity. Rigorous quality control confirms the cell line is free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.

Application

• FOR RESEARCH USE ONLY
  

• The MST4 Knockout 293T Cell Line is a valuable model for studying cell polarity regulation and Hippo signaling pathways. This engineered system enables investigation of MST4’s roles in cytoskeletal reorganization and immune cell migration. Researchers utilize this knockout line to examine disrupted cell polarization processes and altered inflammatory responses. The cell line provides a controlled platform for analyzing kinase-dependent signaling networks in cancer metastasis and autoimmune disorders.