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Tumor Cell Lines

NB-4

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The NB-4 cell line was derived from the marrow of a patient with acute promyelocytic leukemia (APL; M3 in the FAB nomenclature) in second relapse in 1989.
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Product Code

NB4; NB.4

Species

Human

Cat.No

ABC-TC0725

Product Category Tumor Cell Lines
Size/Quantity

1 vial

Shipping Info

Dry Ice

Growth Conditions

37 ℃, 5% CO2

Disease

Leukemia

Biosafety Level

1

Storage

Liquid Nitrogen

Product Type

Human Leukemia Cell Lines

Description

The NB-4 cell line is a human leukemia cell line derived from the bone marrow of a patient with acute promyelocytic leukemia. These cells exhibit a myeloblastic morphology and grow in suspension culture. They carry the t (15;17) (q22;q21) chromosomal translocation, resulting in the PML-RARα fusion gene, which is characteristic of APL. Immunophenotypically, NB-4 cells express granulocytic markers, as well as T-cell markers CD2 and CD4, and the monocytic marker CD9. Upon treatment with retinoic acid, NB-4 cells undergo rapid morphological and functional maturation, making them a valuable model for studying the effects of retinoic acid on hematopoietic proliferation and differentiation. The karyotype of NB-4 cells is hypertriploid with complex chromosomal aberrations, including the t(15;17) translocation. NB-4 cells are not tumorigenic or metastatic in vivo and are not known to be infected with viruses such as HPV, HIV, or EBV.

Citation

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Application

  • The NB4 cell line holds significant value in molecular studies involving the t(15;17) chromosomal translocation. Its ability to undergo functional maturation into polynuclear cells following retinoic acid (RA) treatment is another major advantage. This characteristic makes NB4 cells a valuable tool for examining how RA signaling influences on hematopoietic proliferation and differentiation, specifically in terms of genes regulated by RA (either activated or suppressed). Furthermore, the NB4 cell line can be utilized to explore the effects of different maturation inducers, growth factors, chemotherapeutic drugs, and even retroviral vectors, enabling a broader understanding of their impact on cellular processes.

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