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| Product Code | M-07E; M-O7e; M07-e; M07e; Mo7e; MO7e; M07E; MO7E |
| Species | Human |
| Cat.No | ABC-TC1313 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Disease | Acute Megakaryoblastic Leukemia |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Leukemia Cell Lines |
M-07e cell is a subline of the growth factor-independent cell line M-07, which was established from a 6-month-old girl with acute megakaryoblastic leukemia (AML M7) in 1987. It requires interleukin-3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for growth. M07e cells respond to multiple cytokines and growth factors and exhibit a round, non-adherent morphology in suspension culture, with some cells showing a spindle-like shape and slight adherence. They can become cytokine-independent within 3-4 weeks of culture, likely due to the outgrowth of independent cells.
Why choose M-07e from AcceGen?
AcceGen prepared M-07e cells under strict sterile conditions to prevent contamination from pathogens. They exhibit high viability and display healthy growth based on their morphology. The identity of M07e cells can be confirmed using short tandem repeat (STR) analysis, ensuring their authenticity and consistency in research applications.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
FOR RESEARCH USE ONLY
M-07e cells have important applications in research. They provide a valuable model for studying AML M7, investigating its mechanisms and potential therapies. Additionally, M07e cells are used to explore megakaryocytopoiesis and thrombopoiesis, particularly in relation to the effect of Thrombopoietin (TPO) on platelet production. Moreover, these cells contribute to understanding how cytokines impact cell proliferation, including studying interleukin receptors’ biochemical properties and post-ligand signaling systems. Overall, M-07e cells contribute to research on AML M7, the role of TPO in megakaryocytopoiesis, and the effects of cytokines on cell growth and signaling pathways.
M-07E cells should be cultured in RPMI 1640 medium supplemented with 10-20% fetal bovine serum (FBS), 2mM L-glutamine, and 1% penicillin-streptomycin. Additionally, the medium should be supplemented with 10ng/mL GM-CSF, IL-3, or EPO to support growth. The cells should be maintained at 37°C in a humidified atmosphere with 5% CO₂.
Frequency: Passaging should be done every 2-3 days when the cells reach a density of 2-3×10⁶ cells/mL.
Procedure: Gently resuspend the cells by pipetting up and down.Transfer a portion of the cell suspension to a new culture flask with fresh complete medium supplemented with the appropriate growth factors.
Maintain the recommended cell density by adjusting the split ratio accordingly (usually 1:3 to 1:5).
Cryopreservation: Harvest and centrifuge cells at 300×g for 5 minutes. Resuspend cells in freezing medium (90% FBS and 10% DMSO) at a density of 1-2×10⁶ cells/mL. Aliquot the cell suspension into cryovials and gradually cool to -80°C using a controlled-rate freezing container before transferring to liquid nitrogen for long-term storage.
Thawing: Quickly thaw cryovials in a 37°C water bath. Transfer the cell suspension to a centrifuge tube containing pre-warmed complete medium. Centrifuge at 300×g for 5 minutes to remove DMSO. Resuspend the cell pellet in fresh medium supplemented with the appropriate growth factors and seed into culture flasks.
