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NCI-H460, also referred to as H460, is a cell line originally obtained from a male patient diagnosed with large cell lung carcinoma. These adherent cells exhibit a rapid growth rate, doubling approximately every 33 hours in RPMI 1640 supplemented with 10% FBS. Notably, NCI-H460 cells are capable of forming tumors in both in vitro and in vivo models, including nude mice. The cells demonstrate a conspicuous expression of p53 mRNA, which closely parallels the levels observed in normal lung tissue, while concurrently exhibiting an absence of gross structural DNA abnormalities. These cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein. Isoenzyme analysis reveals unique expression patterns, such as HPRT localized on their surface and varying levels of AK-1, ES-D, Me-2, G6PD, PGM1, and PGM3 isoenzymes. Additionally, NCI-H460 cells possess a hypotriploid karyotype with a modal chromosome number of 57, ranging from 53 to 65.


Why choose NCI-H460 from AcceGen?

NCI-H460 cells from AcceGen exhibit exceptional viability and quality, maintained under sterile conditions with daily monitoring to ensure the absence of bacteria and fungi. They are rigorously tested, confirmed as negative for mycoplasma through PCR-based assays, and authenticated using STR analysis, demonstrating their commitment to stringent quality control measures.

Citation Guide

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).



NCI-H460 cells find versatile applications in cancer and toxicology research, serving as a valuable model for investigating the intricate molecular mechanisms underlying non-small-cell lung cancer. They are particularly beneficial in 3D cell culture systems, aiding in the study of tumor growth and response to treatments. These cells contribute significantly to cancer research, facilitating investigations into potential therapies and drug screenings through high-throughput methods. Moreover, they play a crucial role in toxicology studies, helping assess the impact of various substances on lung cells, which is essential for understanding potential health risks and safety implications.

Growth Conditions

37 ℃, 5% CO2

Product Type

Human Lung Cancer Cell Lines

Product Image AcceGen Frozen Cells & Cell Lines 1 vial
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