NFAT Reporter (Luc) THP-1 Cell Line

Description

NFAT Reporter (Luc) THP-1 Cell Line is a human monocytic reporter line that monitors NFAT (Nuclear Factor of Activated T-cells) signaling via luciferase (Luc) expression. Derived from THP-1 cells, it maintains key native THP-1 characteristics, such as the capacity to upregulate CD14 upon differentiation and PMA-induced differentiation into macrophage-like cells while featuring NFAT-responsive luciferase activity. This system enables sensitive quantification of calcium/calcineurin-NFAT pathway activation, ideal for studying immune responses and drug screening. Cells show dose-dependent bioluminescence upon stimulation while retaining original properties, with stable reporter expression maintained at low passages (<P20). The cells are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, Fungi, Yeast and Bacteria.

Application

• FOR RESEARCH USE ONLY
  

• The NFAT Reporter (Luc) THP-1 Cell Line is a genetically engineered human monocytic model designed to monitor nuclear factor of activated T cells (NFAT) signaling activity through luciferase reporter expression. This system enables quantitative detection of calcium-dependent NFAT activation in response to immunomodulators, T-cell receptor agonists, or calcineurin inhibitors via bioluminescence imaging. The cell line provides dynamic readouts for studying immune cell activation, cytokine production, and immunosuppressant mechanisms while maintaining native THP-1 characteristics. Maintain in RPMI-1640 with 10% FBS and selection antibiotics to ensure stable reporter performance during investigations of calcium-NFAT signaling pathways.