Description
The NF-KB/Rel signaling pathway includes NF-KB2 p52/p100, NF-KB1 p50/p105, c-Rel, RelA/p65, and RelB. These proteins act as dimeric transcription factors, controlling gene regulation in a wide range of biological processes, including innate and adaptive immunity, inflammation, stress response, B cell development, and lymphoid organ development. In the classical or non-classical pathways, NF-KB/Rel proteins are bound and inhibited by IKB proteins. Pro-inflammatory cytokines, LPS, growth factors, and antigen receptor activation stimulate the IKK complex (IKKβ, IKKα, and NEMO) to phosphorylate IKB proteins. Phosphorylation of IKB leads to its ubiquitination and proteasomal degradation, releasing the NF-KB/Rel complex. The active NF-KB/Rel complex is further activated by phosphorylation and translocated to the nucleus, inducing the expression of target genes.The NFKB Reporter THP1 Cell Line uses the THP-1 cell line as a tool cell, stably expressing the NFKB-Luc reporter gene through lentivirus infection, and can be used to detect NFKB signaling transduction. Upon activation by pro-inflammatory cytokines or lymphokine receptor agonists, endogenous NF-KB transcription factors bind to DNA response elements, inducing the transcription of luciferase reporter genes.
| Species |
Human
|
| Cat.No |
ABC-RC145F
|
| Quality Control |
All cells test negative for mycoplasma, bacteria, yeast, and fungi.
|
| Product Category |
Transfected Stable Cell Lines |
| Size/Quantity |
1 vial
|
| Cell Type |
Monocyte
|
| Growth Mode |
Suspension
|
| Shipping Info |
Dry Ice
|
| Growth Conditions |
37 ℃, 5% CO2
|
| Source Organ |
Peripheral blood
|
| Disease |
Acute monocytic leukemia
|
| Biosafety Level |
1
|
| Storage |
Liquid Nitrogen
|
| Product Type |
Reporter Stable Cell Lines
|
| Host Cell |
THP1
|
