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| Product Code | OCI-Aml-3; OCI/AML-3; OCI-AML-3; OCI/AML3; OCI AML3; OCIAML3 |
| Species | Human |
| Cat.No | ABC-TC179D |
| Quality Control | All cells test negative for mycoplasma, bacteria, yeast, and fungi. |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Peripheral Blood |
| Disease | Acute Myeloid Leukemia |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Acute Myeloid Leukemia Cell Lines |
OCI-AML3 is a human acute myeloid leukemia (AML) cell line that was originally isolated from the peripheral blood of a 57-year-old man with acute myeloid leukemia (AML FAB M4). OCI-AML3 exhibits lymphoblast-like morphology and suspension culture properties. The stemline karyotype model number of OCI-AML3 is 48 with a range from 45 to 50. This cell line is hyperdiploid; it displays chromosomal counts within the hyperdiploid range. OCI-AML3 carries a mutation in the nucleophosmin 1 (NPM1) gene and DNMT3A gene. NPM1 is considered an ideal biomarker for AML.
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The OCI-AML3 cell line is a valuable model for studying the biology of acute myeloid leukemia (AML) and the underlying molecular mechanisms, supporting the development of potential treatments such as cytotoxic chemotherapy and cell therapy. It is also commonly used to evaluate the efficacy of new drugs, aiding in the identification of promising therapeutic candidates.
1. The recommended culture medium for OCI-AML3 is RPMI 1640 + 20%FBS.
Preheat the complete culture medium in a 37℃ water bath for 30 minutes.
Use a pipette to draw 6-7 mL of complete culture medium into a 15 mL centrifuge tube in a clean bench.
2. Then take the frozen cells out of the liquid nitrogen or dry ice. When reviving, shake them slightly to remove the residual dry ice or liquid nitrogen, then quickly clamp the lid with tweezers and place it in a 37℃ water bath and shake it quickly (note: the water cannot cover the lid), so that it completely melts in about 1 minute.
3. In the clean bench, wipe the outer wall of the cryotube with an 75% alcohol to disinfect it and let it dry slightly. Use a single-channel pipette to transfer all thawed cell suspensions to the complete culture medium prepared in advance, cover the lid, and centrifuge at 1100rpm for 4 minutes at room temperature to collect cells.
4. Carefully aspirate the supernatant in the clean bench, use a single-channel pipette to absorb 1 mL of fresh complete culture medium to resuspend the cells into a single cell suspension, and then transfer to a T25 culture flask (or 6cm dish) containing 5 mL of complete culture medium.
Note: Do not directly recover to a T75 flask or a 10cm dish.
OCI-AML3 was established from the peripheral blood of a 57-year-old man diagnosed with acute myeloid leukemia (AML). The cells have specific genetic mutations that are representative of AML, carrying both NPM1 gene mutation and the DNMT3A R882C mutation. The NPM1 gene mutation is of type A, which refers to mutations that involve the nucleophosmin 1 (NPM1) gene.
The doubling time of OCI-AML3 cells is approximately 28-40 hours, though this can vary depending on culture conditions.
