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Tumor Cell Lines

ONS-76

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Human medulloblastoma cell line. Positive for 145 kDa and 200 kDa neurofilament and NSE. Negative for GFAP and S-100 protein.
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Product Code

ONS76

Species

Human

Cat.No

ABC-TC0875

Product Category Tumor Cell Lines
Size/Quantity

1 vial

Shipping Info

Dry Ice

Growth Conditions

37 ℃, 5% CO2

Source Organ

Brain

Biosafety Level

1

Storage

Liquid Nitrogen

Product Type

Human Nerve Tumors Cell Lines

Description

ONS-76 cell
The ONS-76 cell line is a human medulloblastoma tumor cell line derived from cerebellar tumor tissue of a 2-year-old Japanese girl. Classified as a neuronal tumor line, it exhibits polygonal morphology. These cells show features of undifferentiated and early-differentiating neural stem-like cells, with high proliferation and a semi-differentiated state. Genetically, ONS-76 carries wild-type p53 and expresses neuron-specific markers like NSE and neurofilament proteins, while lacking glial markers GFAP and S-100. Upon interferon-gamma stimulation, the cells express class I and II MHC antigens. ONS-76 also shows elevated VE-cadherin (CDH5) and includes CD133-positive subpopulations, suggesting stem-like traits. These cells are tumorigenic in vivo. ONS-76 is widely used to study medulloblastoma radioresistance, neural progenitor differentiation, and therapeutic targeting in neuro-oncology.
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Citation

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Application

  • ONS-76 has found multiple applications in research and scientific investigations. It serves as a valuable model for conducting in vitro drug sensitivity assays with different antitumor agents against tumor cell lines. Furthermore, ONS-76 is utilized to explore the radio-resistance of medulloblastoma, aiding in understanding the response of these tumor cells to radiation treatment. Additionally, ONS-76 is employed to investigate the role of MHC class II antigens in the immune response, particularly on human medulloblastoma cells with neuronal differentiation. Lastly, this cell line contributes to studying the mechanisms involved in the differentiation of immature bipotential cells into both neuronal and glial cells, shedding light on the intricate process of cell development and differentiation.

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