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Polybrene(hexadimethrine bromide) (10 mg/mL)

  • For research use only

Cat No.

ABC-BR0010

Polybrene by AcceGen enhances viral & non-viral transfection efficiency, aids protein sequencing, and acts as a heparin antagonist in bioprocessing.

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  • Polybrene(hexadimethrine bromide) (10 mg/mL)
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Home | Cell Culture Media, Kits & Reagents | Cell Biology Reagents | Polybrene(hexadimethrine bromide) (10 mg/mL)

Description

Polybrene (Hexadimethrine Bromide) is a cationic polymer widely used to enhance the efficiency of DNA transfection in mammalian cells, particularly in conjunction with lipid-based methods and reverse or lentiviral transduction. It functions by neutralizing electrostatic repulsion between viral particles and cell surface sialic acids, thereby promoting viral adsorption and cellular uptake. Beyond virology, Polybrene serves as an effective heparin antagonist for inducing nonspecific erythrocyte agglutination. It is also employed in protein sequencing, where low concentrations significantly reduce peptide degradation during automated Edman degradation, and can improve binding affinity when applied to PVDF membranes in blotting applications.

Cat.No

ABC-BR0010
Product Category

Cell Culture Media, Kits & Reagents
Size/Quantity

1 kit
Shipping Info

Ice Pack
Storage

-20°C

Application

  • Polybrene (hexadimethrine bromide) is widely utilized to enhance the efficiency of viral transduction, particularly for lentiviral and retroviral gene delivery, by neutralizing electrostatic repulsions between viral particles and cell membranes to promote viral adhesion and entry. Its stable performance under 2–8°C storage ensures reliable long-term functionality. With no complex pretreatment required, it can be directly added to virus-containing media, streamlining experimental procedures while significantly improving infection rates across diverse mammalian cell types.

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High Viability
To succeed in cell culture
Precision and Reliability
To support a consistent result
Customization Options
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