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Primary Cells

Rat Colonic Epithelial Cells

  • For research use only

Cat No.

ABC-TC4113
Product Type

Rat Primary Cells
Cell Type

Epithelial
Species

Rat
Growth Conditions

37 ℃, 5% CO2
Source Organ

Colonic
Disease

Normal
Storage

Liquid Nitrogen

Rat Primary Colonic Epithelial Cells from AcceGen are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats.

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  • Rat Colonic Epithelial Cells
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Description

Rat Primary Colonic Epithelial Cells from AcceGen are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Primary Colonic Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in AcceGen’s Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1×10^6 cells and is delivered frozen.
Rat Primary Colonic Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Primary Colonic Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended.
Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Species

Rat
Cat.No

ABC-TC4113
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Product Category Primary Cells
Size/Quantity

1 vial
Cell Type

Epithelial
Growth Mode

Adherent
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Colonic
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Rat Primary Cells

Application

  • For research use only

Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Frequently Asked Questions

  • What is the primary function of colonic epithelial cells in the rat colon?

    These cells form a protective barrier in the colon, involved in nutrient absorption and immune defense.

  • How are Rat Colonic Epithelial Cells typically isolated?

    They are usually isolated from rat colon tissue using enzymatic digestion followed by mechanical dissociation.

  • In what types of studies are Rat Colonic Epithelial Cells commonly used?

    They are often used in studies related to gut health, inflammation, cancer research, and barrier function.

  • What are the optimal culture conditions for Rat Colonic Epithelial Cells?

    These cells should be cultured in a specialized medium for epithelial cells, maintained at 37°C with 5% CO2, typically on a collagen or fibronectin-coated surface.

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