SIRT4 Knockout 293T Cell Line

Description

SIRT4 Knockout 293T Cell Line is generated from human embryonic kidney 293T cells through CRISPR/Cas9-mediated disruption of the Sirtuin 4 (SIRT4) gene, a mitochondrial NAD⁺-dependent deacylase involved in energy homeostasis and metabolic regulation. This knockout model exhibits altered mitochondrial metabolism while maintaining the characteristic adherent growth, epithelial morphology, and high transfection efficiency of 293T cells. It serves as a valuable research tool for investigating mitochondrial function, lipid metabolism, glutamine regulation, insulin secretion, and cancer metabolism. The knockout efficiency is validated through genomic PCR, Sanger sequencing, and Western blot analysis. The cell line is maintained at low passage numbers (<P20) to ensure genetic stability and has been rigorously tested free of HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.