SLVL

SLVL (VL51) is a human B-cell lymphoma cell line derived from the spleen of a middle-aged female patient diagnosed with splenic lymphoma with circulating villous lymphocytes. The cells exhibit a lymphocyte-like morphology with partial adherence. They grow under standard conditions (37°C, 5% CO₂) in RPMI-1640 medium supplemented with 10% FBS. SLVL cells display a committed B-cell immunophenotype, expressing CD10, CD19, CD20, and surface immunoglobulins, while maintaining monoclonality confirmed by IgH and TCRβ gene rearrangements. These cells are EBNA-positive, indicating transformation by Epstein-Barr virus (EBV). They are negative for HIV-1/2, HBV, HCV, mycoplasma, bacteria, yeast, and fungi, ensuring biosafety. Cryopreserved in liquid nitrogen and typically shipped on dry ice, SLVL cells are ideal for research into leukemogenesis, B-cell malignancies, and splenic lymphoma pathogenesis.