| Species |
Human
|
| Cat.No |
ABC-RC045Y
|
| Quality Control |
All cells test negative for mycoplasma, bacteria, yeast, and fungi.
|
| Product Category |
Transfected Stable Cell Lines |
| Size/Quantity |
1 vial
|
| Cell Type |
Monocyte
|
| Shipping Info |
Dry Ice
|
| Growth Conditions |
37 ℃, 5% CO2
|
| Source Organ |
Peripheral Blood
|
| Disease |
Acute Monocytic Leukemia
|
| Biosafety Level |
1
|
| Storage |
Liquid Nitrogen
|
| Product Type |
Reporter Stable Cell Lines
|
| Host Cell |
THP-1
|
| Gene Info |
GFP / Luciferase
|
Description
THP-1/GFP-Luciferase Stable Cell Line is a dual-reporter human monocytic cell line engineered from THP-1 cells through stable co-expression of green fluorescent protein (GFP) and luciferase (Luc), the cell line maintains key native THP-1 characteristics, such as the capacity to upregulate CD14 upon differentiation and PMA-induced differentiation into macrophage-like cells. This versatile reporter system combines the advantages of fluorescent visualization for cellular localization studies with sensitive bioluminescent detection for quantitative cell tracking, making it particularly valuable for immunological research, cancer microenvironment studies, and cell therapy development. The GFP marker enables real-time monitoring of cell migration and interactions through fluorescence imaging, while the luciferase activity permits highly sensitive in vitro and in vivo quantification of cell proliferation and viability using bioluminescence imaging (BLI) technology. Cells are maintained at low passage numbers (<P20) to ensure stable expression of both reporters and consistent experimental performance. The cells are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, Fungi, Yeast and Bacteria.
