IRF Reporter (Luc) THP-1 Cell Line

Description

IRF Reporter (Luc) THP-1 Cell Line is a genetically engineered human monocytic cell line designed to monitor interferon regulatory factor (IRF) signaling pathway activation through luciferase (Luc) reporter expression. Derived from THP-1 cells, it maintains key native THP-1 characteristics, such as the capacity to upregulate CD14 upon differentiation and PMA-induced differentiation into macrophage-like cells while featuring a luciferase reporter system under the control of IRF-responsive elements. The cell line enables sensitive quantification of innate immune responses through bioluminescence measurement, making it particularly valuable for studying viral infection responses, TLR signaling, and interferon production. Cells exhibit dose-dependent luciferase activity upon IRF pathway activation while retaining their original biological properties, allowing for high-throughput screening of immunomodulatory compounds. Maintained at low passage numbers (<P20) to ensure reporter stability and consistent performance, this cell line provides a reliable tool for immunology and virology research. The cells are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, Fungi, Yeast and Bacteria.