THP-1/RFP-Luciferase Stable Cell Line

Description

THP-1/RFP-Luciferase Stable Cell Line is a dual-reporter human monocytic cell line engineered from THP-1 cells through stable co-expression of red fluorescent protein (RFP) and luciferase (Luc). The cell line maintains key native THP-1 characteristics, such as the capacity to upregulate CD14 upon differentiation and PMA-induced differentiation into macrophage-like cells, while providing both fluorescent and bioluminescent tracking modalities. The RFP marker enables real-time visualization of cell localization and migration, while the luciferase reporter allows sensitive quantitative detection of cell proliferation and viability in vitro and in vivo. Particularly valuable for immunological studies and cancer research, this dual-reporter system permits cross-validation between fluorescence microscopy and bioluminescence imaging (BLI) techniques. Cells exhibit stable, high-level expression of both reporters when maintained at low passage numbers (<P20), ensuring experimental reproducibility. The cells are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, Fungi, Yeast and Bacteria.

Application

• FOR RESEARCH USE ONLY
  

• The THP-1/RFP-Luciferase Stable Cell Line is a dual-reporter human monocytic model combining red fluorescent protein (RFP) and luciferase expression for multimodal cell tracking. This system enables both fluorescence-based cellular localization (RFP) and sensitive bioluminescence quantification (luciferase), providing complementary detection methods for studying monocyte migration, immune responses, and cell therapies. The cell line maintains THP-1 differentiation potential while offering stable dual-reporter expression. Culture in RPMI-1640 with 10% FBS and selection antibiotics ensures consistent performance in both in vitro and in vivo applications.