THP-1/STAT5 Reporter (Luc) Stable Cell Line

Description

THP-1/STAT5 Reporter (Luc) Stable Cell Line is a human monocytic cell line engineered to monitor STAT5 signaling through luciferase (Luc) expression. Derived from THP-1 cells, it maintains key native THP-1 characteristics, such as the capacity to upregulate CD14 upon differentiation and PMA-induced differentiation into macrophage-like cells, while featuring STAT5-responsive luciferase activity. This reporter system enables sensitive quantification of STAT5 activation by cytokines (IL-2, IL-7, GM-CSF), making it ideal for immunology and hematopoiesis research. The cell line exhibits dose-dependent bioluminescence suitable for high-throughput screening, with stable reporter expression maintained at low passages (<P20). The cells are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, Fungi, Yeast and Bacteria.

Application

• FOR RESEARCH USE ONLY
  

• The THP-1/STAT5 Reporter (Luc) Stable Cell Line is a human monocytic cell model engineered to monitor STAT5 signaling activity through luciferase reporter expression. This system enables sensitive, quantitative detection of STAT5 pathway activation in response to cytokines (e.g., IL-2, GM-CSF) or JAK-STAT inhibitors via bioluminescence imaging. The cell line retains native THP-1 characteristics while providing dynamic readouts for immunology research, leukemia studies, and drug screening applications. Maintain in RPMI-1640 with 10% FBS and selection antibiotics to ensure stable reporter performance during hematopoietic signaling investigations.