Tie2 HEK-293 Cell Line

Frequently Asked Questions

• What are the culture characteristics of this cell line?

  This Tie2 stable line is adherent, like parental HEK-293 cells. No special coating is required under routine culture. For certain experiments (e.g., ELISA or phosphorylation assays), surface coatings such as poly-L-lysine may be used to improve consistency, but pre-testing is recommended.

• What medium and selection pressure are recommended?

  Culture in DMEM + 10% FBS + 1% P.S + 0.75 µg/mL puromycin. Continuous selection with puromycin is recommended to maintain stable Tie2 expression. During 1–2 passages before functional assays, puromycin can be reduced or removed to avoid potential effects on cell physiology.

• What Tie2 expression data are available?

  Tie2 surface expression has been confirmed via flow cytometry.

• Is functional validation available?

  Systematic validation of Tie2 phosphorylation upon Ang1/Ang2 stimulation or downstream signaling has not yet been performed for this line.

• What is the stability and QC information?

  Supplied cells are Pn+5; expression is expected to be stable for over 20 passages. COA documents include STR profiling, mycoplasma testing, and post-thaw viability. Low-passage cells should be frozen for long-term use.

• How was the Tie2 construct integrated?

  Supplied cells are Pn+5; expression is expected to be stable for over 20 passages. COA documents include STR profiling, mycoplasma testing, and post-thaw viability. Low-passage cells should be frozen for long-term use.