YTHDF3 Knockout 293T Cell Line

Description

YTHDF3 Knockout 293T Cell Line is engineered from human embryonic kidney 293T cells through CRISPR/Cas9-mediated disruption of the YTH N6-Methyladenosine RNA Binding Protein 3 (YTHDF3) gene, a critical m6A-binding protein that modulates mRNA translation efficiency and works cooperatively with other YTHDF proteins to regulate RNA metabolism. This knockout model exhibits altered translation dynamics of m6A-modified transcripts while maintaining 293T cells’ characteristic adherent growth with epithelial morphology and high transfection efficiency. The cell line provides a powerful tool for studying epitranscriptomic regulation, particularly in research involving viral infection responses, cancer immunotherapy targets, and neural development where YTHDF3 mediates the dual regulation of mRNA stability and translation. Maintained at low passage numbers (<P20) with genetic stability, the knockout efficiency is validated through triple verification (genomic PCR, Sanger sequencing, and m6A-IP qPCR), demonstrating impaired binding to m6A-modified RNAs. The cell line has undergone rigorous quality control and is certified free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.