ZDHHC1 Knockout 293T Cell Line

Description

ZDHHC1 Knockout 293T Cell Line is engineered from human embryonic kidney 293T cells using CRISPR/Cas9 technology to disrupt the Zinc Finger DHHC-Type Containing 1 (ZDHHC1) gene, a palmitoyltransferase that mediates protein S-palmitoylation and regulates membrane trafficking, signal transduction, and synaptic function. This knockout model exhibits impaired protein palmitoylation while maintaining 293T cells’ characteristic adherent growth with epithelial morphology and high transfection efficiency. The cell line provides a valuable tool for studying post-translational modifications, neuronal development, and immune responses, particularly in research involving T-cell activation, neurotransmitter receptor localization, and viral entry mechanisms. Maintained at low passage numbers (<P20) with genetic stability, the knockout efficiency is validated through genomic PCR, Sanger sequencing, and acyl-biotin exchange assays demonstrating defective palmitoylation activity. The cell line has undergone rigorous quality control and is certified free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.