Tumor Cell Lines

3T3 clone A31

  • For research use only

Cat No.

ABC-TC0010

Product Type

Mouse Embryo Cell Lines

Cell Type

Fibroblast

Species

Mouse

Growth Conditions

37 ℃, 5% CO2

Source Organ

Embryo

Disease

Normal

Product Code

BALB/c 3T3 clone A31; Balb/c3T3; BALB/c 3T3; Balb/c 3T3; BALB/3T3; Balb/3T3-4-Cl31; BALB/3T3 cl. A31; BALB 3T3 clone A31; BALB/3T3 (clone A31); B/C3T3; 3T3-A31; 3T3(A31); A31; A31N

Mouse BALB/c embryo. Cells have now lost their contact inhibition.

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Description

3T3 clone A31 is a spontaneously immortalized murine fibroblast cell line derived from BALB/c mouse embryo tissue through serial passage using the 3T3 protocol. This clone was selected from the parental BALB/c 3T3 population for its uniform morphology and reproducible growth kinetics. The cells exhibit elongated fibroblast-like morphology and grow as adherent monolayers with high proliferation rates. A hallmark of A31 cells is their loss of contact inhibition, allowing continued growth beyond confluence—a feature associated with early stages of transformation. The karyotype is pseudodiploid with moderate chromosomal instability, and the cells are non-tumorigenic under standard conditions. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.

Product Code

BALB/c 3T3 clone A31; Balb/c3T3; BALB/c 3T3; Balb/c 3T3; BALB/3T3; Balb/3T3-4-Cl31; BALB/3T3 cl. A31; BALB 3T3 clone A31; BALB/3T3 (clone A31); B/C3T3; 3T3-A31; 3T3(A31); A31; A31N

Species

Mouse

Cat.No

ABC-TC0010

Product Category

Tumor Cell Lines

Size/Quantity

1 vial

Cell Type

Fibroblast

Growth Mode

Adherent

Shipping Info

Dry Ice

Growth Conditions

37 ℃, 5% CO2

Source Organ

Embryo

Disease

Normal

Biosafety Level

1

Storage

Liquid Nitrogen

Product Type

Mouse Embryo Cell Lines

Application

  • 3T3 clone A31 cells are a foundational model in cancer research, toxicology, and cellular transformation studies. Their susceptibility to chemical mutagens and oncogene transfection makes them widely used in focus formation assays, anchorage-independent growth evaluation, and Ras or Src pathway activation studies. Additionally, they are employed in screening tumor-promoting agents and evaluating carcinogenicity in vitro.

Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

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High Viability
To succeed in cell culture
Precision and Reliability
To support a consistent result
Customization Options
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