For research use only
| Cat No. | ABC-TC0011 |
| Product Type | Mouse Embryo Cell Lines |
| Cell Type | Fibroblast |
| Species | Mouse |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Embryo |
| Disease | Normal |
| Product Code | 3T3-L1; 3T3L1; 3T3-L1 ad; NIH-3T3-L1; NIH3T3-L1 |
3T3 L1 is a continuous strain of 3T3 developed through clonal isolation.
3T3-L1 cell line is a continuous murine preadipocyte cell line derived from Swiss mouse embryonic fibroblasts via clonal isolation, notable for its loss of contact inhibition. Under adipogenic induction, the cells differentiate into lipid-laden, adipocyte-like cells, accumulating lipid droplets and expressing adipose-specific genes. These cells are spindle-shaped when undifferentiated and adopt rounded, lipid-filled morphology upon induction. Transcriptomic profiling has confirmed upregulation of metabolic pathways and mitochondria-related genes during differentiation. Cytogenetically, 3T3-L1 retains a near-tetraploid karyotype typical of NIH-3T3 derivatives. The line is non-tumorigenic. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.
| Product Code | 3T3-L1; 3T3L1; 3T3-L1 ad; NIH-3T3-L1; NIH3T3-L1 |
| Species | Mouse |
| Cat.No | ABC-TC0011 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Fibroblast |
| Growth Mode | Adherent |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Embryo |
| Disease | Normal |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Mouse Embryo Cell Lines |
3T3-L1 cells are a cornerstone model for adipogenesis and metabolic disease research. They are widely used to study lipid accumulation, adipocyte gene regulation, and insulin signaling. Recent studies employ 3T3-L1 to investigate the effects of nutrients, cytokines, or bioactive compounds on differentiation and browning processes, including analysis of mitochondrial function, lysosomal activity, and adipogenic transcription factors like PPARγ and C/EBPs. They also serve in co-culture systems to evaluate adipocyte–tumor cell interactions.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
