For research use only
| Cat No. | ABC-TC0037 |
| Product Type | Mouse Skin Cell Lines |
| Cell Type | Fibroblast |
| Species | Mouse |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Adipose |
| Disease | Normal |
| Product Code | A-9; AG 9 |
Explore A9 mouse cell line derived from L929 cells, partially resistant to azaguanine, ideal for genetic research and enzyme activity studies.
A9 is a murine fibroblast tumor cell line derived from areolar and adipose tissues of C3H/An mice and represents a subline of the L929 parental line. This chemical selection conferred partial resistance to 8-azaguanine and altered purine metabolism pathways. A9 cells retain fibroblast-like, elongated morphology and adhere tightly in monolayer culture. While not spontaneously tumorigenic, A9 is frequently used in tumorigenesis assays via transformation or fusion due to its high proliferative capacity and altered nucleotide metabolism. Cytogenetically, A9 exhibits stable murine karyotypes with structural rearrangements consistent with L929 derivation. The line is widely used in somatic cell genetics, gene transfer, and hybridoma construction. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.
| Product Code | A-9; AG 9 |
| Species | Mouse |
| Cat.No | ABC-TC0037 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Fibroblast |
| Growth Mode | Adherent |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Adipose |
| Disease | Normal |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Mouse Skin Cell Lines |
A9 cells are a key tool in somatic cell genetics and molecular biology, especially in gene transfer, cell fusion, and selection studies using azaguanine or HAT media. Their partial resistance to 8-azaguanine and altered purine metabolism pathways make them suitable for experiments involving HGPRT selection or drug resistance screening. A9 is frequently employed as a fusion partner in hybridoma technology and in research exploring chromosomal instability, purine salvage pathways, and cell cycle regulation. Due to their stable fibroblastic phenotype and predictable response to selection agents, A9 cells are well-suited for high-efficiency gene expression and functional genomics applications in murine models.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
