B2M Knockout THP-1 Cell Line

Description

B2M Knockout THP-1 Cell Line is derived from the human monocytic THP-1 cell line, which serves as the host cell, and was generated via CRISPR/Cas9-mediated gene editing to disrupt the Beta-2-microglobulin (B2M) gene. THP-1 cells are suspension-grown immune cells with round morphology, commonly used to study monocyte/macrophage functions, immune responses, and inflammatory pathways. This knockout cell line exhibits loss of B2M expression, a key component of MHC class I molecules, making it valuable for research in immune evasion, antigen presentation, and immunotherapy. The edited cells are maintained at low passage numbers (<P20) to ensure genetic stability, and knockout efficiency is validated at the gene or protein level through PCR, Sanger sequencing, or Western blot. The cells are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.