For research use only
| Cat No. | ABC-TC0068 |
| Product Type | Mouse Mouseculus Fibroblas Lymphocytes Cell Lines |
| Cell Type | Fibroblast |
| Species | Mouse-Embryo, 14 To Mouse – 17 Days Old, Mus Musculus |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Embryo |
| Product Code | BALB/c 3T3 clone A31; Balb/c3T3; BALB/c 3T3; Balb/c 3T3; BALB/3T3; Balb/3T3-4-Cl31; 3T3 clone A31; BALB/3T3 cl. A31; BALB 3T3 clone A31; BALB/3T3 (clone A31); B/C3T3; 3T3-A31; 3T3(A31); A31; A31N |
Explore BALB/3T3 clone A31 for transformation assays, fibroblast biology, and cell proliferation studies in standardized research culture conditions.
BALB/3T3 clone A31 is a murine fibroblast-like cell line derived from the primary embryonic tissue of a BALB/c mouse. Developed through repeated subculturing of 3T3 cells under defined conditions, the A31 clone is known for its stable growth kinetics and contact inhibition properties. These cells display an elongated fibroblast morphology and grow as an adherent monolayer, forming a uniform, contact-inhibited sheet in confluent cultures. Cytogenetic analysis reveals a near-diploid karyotype with occasional chromosomal abnormalities, supporting their use in transformation and oncogenicity assays. While not inherently tumorigenic, A31 cells are highly susceptible to transformation by chemical carcinogens or oncogenes, and have been extensively employed in cellular transformation and anchorage-independent growth assays. The cells undergo stringent screening and testing procedures to ensure they are free from contamination by Mycoplasma, fungi, yeast, and bacteria.
| Product Code | BALB/c 3T3 clone A31; Balb/c3T3; BALB/c 3T3; Balb/c 3T3; BALB/3T3; Balb/3T3-4-Cl31; 3T3 clone A31; BALB/3T3 cl. A31; BALB 3T3 clone A31; BALB/3T3 (clone A31); B/C3T3; 3T3-A31; 3T3(A31); A31; A31N |
| Species | Mouse-Embryo, 14 To Mouse – 17 Days Old, Mus Musculus |
| Cat.No | ABC-TC0068 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Fibroblast |
| Growth Mode | Adherent |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Embryo |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Mouse Mouseculus Fibroblas Lymphocytes Cell Lines |
BALB/3T3 clone A31 cells are a well-established model in transformation assays used to study the carcinogenic effects of chemicals, viral oncogenes, and radiation exposure. Their defined growth behavior and sensitivity to oncogenic transformation make them ideal for studies in tumor initiation, proliferation, and anchorage-independent growth. These cells are also widely used in toxicology, cell cycle regulation, and gene function analysis involving Ras, Myc, and p53 signaling.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
All Balb/c 3T3 Clone A31 cells in our inventory are maintained at passage number 5 or below. We make every effort to supply cells with the lowest possible passage number to ensure optimal performance and stability.
