C57BL/6 Mouse Intestinal Epithelial Cells

Description

C57BL/6 Mouse Intestinal Epithelial Cells are primary cells isolated from pathogen-free C57BL/6 mice, a widely used inbred strain with a fully sequenced genome. Cryopreserved at early passage, these cells maintain high viability and plating efficiency. They contribute to gut homeostasis and nutrient absorption and play an essential role in intestinal immune regulation by secreting cytokines and chemokines, supporting epithelial cell differentiation and expressing major histocompatibility complex class II (MHC II) involved in immune surveillance and the C57BL/6 immune response. Through close interactions with luminal factors, these epithelial cells serve as a critical interface between the host and the gut microbiome, making them highly relevant for studying gut microbiome and epithelial cell interactions as well as host–microbiota relationships associated with the C57BL/6 gut microbiota. Owing to the well-characterized genetic background of the C57BL/6 strain, these cells are widely used in reproducible intestinal inflammation mouse models and related genetic and disease modeling studies. These cells are non-proliferative in vitro and cannot be passaged. To preserve their functional integrity, repeated freeze–thaw cycles should be avoided.