+1 862-686-2696
Featured Products
Primary Cells
Immortalized Cell Lines
Stem Cells
Tumor Cell Lines
Transfected Stable Cell Lines
Exosomes
MicroRNA Agomir/Antagomir
Cell Culture Media, Kits & Reagents
Explore Products
Cell
Cell
MicroRNA
MicroRNA
Test and Analysis
Test and Analysis
Immortalized Cell Lines

Immortalized Mouse Kupffer Cell Line (ImKC)

  • For research use only

Cat No.

ABI-TC192D
Product Type

Immortalized Cell Line
Cell Type

Kupffer Cell
Species

Mouse
Growth Conditions

37 ℃, 5% CO2
Source Organ

Liver
Disease

Normal
Storage

Liquid Nitrogen

Immortalized Mouse Kupffer Cell Line (ImKC) enables liver immunology research, macrophage function studies, and inflammation modeling in hepatic systems.

Product Image
  • Brightfield micrograph of Immortalized Mouse Kupffer Cell Line (ImKC), showing adherent growth, 4X magnification.
  • Immortalized Mouse Kupffer Cell Line (ImKC)
Get a Quote
Home | Immortalized Cell Lines | Immortalized Mouse Kupffer Cell Line (ImKC)

Description

Immortalized Mouse Kupffer Cells (ImKCs) are derived from primary Kupffer cells—the liver-resident macrophages of mouse liver—selected using the F4/80 surface marker, a hallmark of Kupffer cells. The cell line was immortalized via lentiviral transduction of the SV40 Large T antigen, leading to increased telomerase activity and elevated p53 expression while retaining key functional properties of primary Kupffer cells, including phagocytosis, antigen presentation, and immune surveillance. ImKCs are widely used in biochemical assays such as PCR, Western blotting, and immunoprecipitation for immunological and liver-related research. When cultured in optimized Mouse Kupffer Cell Medium (e.g., AcceGen’s formulation), they can be passaged multiple times while maintaining stability. Importantly, ImKCs remain non-tumorigenic and non-metastatic in vivo. The cells are cryopreserved to ensure high viability and functionality upon thawing. As an immortal cell line derived from immortal mouse Kupffer cells, this kupffer cell line serves as a representative mouse cell line model for liver immunology and macrophage-related research

Product Code

Immortalized Mouse Kupffer Cell Line (ImKC), Immortalized Mouse Kupffer Cells, ImKC, Murine Kupffer Cell Line
Species

Mouse
Cat.No

ABI-TC192D
Product Category

Immortalized Cell Lines
Size/Quantity

1 vial
Cell Type

Kupffer Cell
Growth Mode

Adherent
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Liver
Disease

Normal
Biosafety Level

2
Storage

Liquid Nitrogen
Product Type

Immortalized Cell Line
Immortalization Method

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen.
Morphology

Macrophage-like
Quality Control

All cells are tested negative for mycoplasma, bacteria, yeast, and fungi.

Application

  • Immortalized Mouse Kupffer Cells (ImKC) provide a powerful tool for investigating liver immune functions, macrophage biology, and hepatic disease mechanisms. These cells, with their authentic macrophage-like morphology and retained phagocytic and immune surveillance abilities, serve as an excellent model for studying Kupffer cell behavior in vitro. Their high telomerase activity and stable expression of immune markers make them perfect for exploring inflammatory responses, innate immunity, and pathogen interactions. They offer a reliable system for drug screening, toxicology assays, and modeling liver-related diseases including fibrosis and hepatitis. Cryopreserved for high viability and functionality, these cells are an indispensable asset for researchers diving deep into liver immunology and macrophage-mediated pathophysiology.

Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
Dong T, Hu G, Fan Z, et al. Activation of GPR3-β-arrestin2-PKM2 pathway in Kupffer cells stimulates glycolysis and inhibits obesity and liver pathogenesis. Nature Communications. 2024;15(1):807. doi:10.1038/s41467-024-45167-5

Frequently Asked Questions

  • Can Immortalized Mouse Kupffer Cell Line (ImKC) be cryopreserved, thawed, and reused multiple times like a standard cell line?

    Yes, our immortalized cells can be cryopreserved and recovered just like established cell lines. Under appropriate culture conditions, ImKC cells can be passaged at least 10 times or more after thawing under AcceGen’s recommended culture conditions.

  • Online Inquiry
  • Product Reviews

Inquiring Immortalized Mouse Kupffer Cell Line (ImKC)

We know how valuable your research is to you, but are you wondering what you can expect to pay for quick accurate results every time? Fill out a request in the form below and we’ll get back to you within 24 hours with a quote.
High Viability
To succeed in cell culture
Precision and Reliability
To support a consistent result
Customization Options
Tailed to your research

Tags

Let's Advance Life Science Research Together

Contact Us
AcceGen Scroll Top Button