C57BL/6 Mouse Intestinal Smooth Muscle Cells

Description

C57BL/6 Mouse Intestinal Smooth Muscle Cells are primary cells isolated from the intestinal tissue of pathogen-free laboratory mice, serving as a classic model for investigating smooth muscle function in mouse gut. These intestinal muscle cells reside within the muscular layers of the intestinal wall, specifically in the inner circular muscle layer and the outer longitudinal muscle layer. Following primary culture, these cells are cryopreserved. The cells regulate peristaltic movements through Ca2+-dependent contraction, maintain intestinal wall structural integrity and actively facilitate immune modulation through cytokine secretion. Studies have demonstrated their involvement in intestinal obstruction, inflammatory bowel disease, and fibrotic strictures via TGF-β1/Smad3-driven collagen deposition. Phenotypic validation is confirmed by α-smooth muscle actin (α-SMA), demonstrating their contractile function. For optimal performance, repeated freezing and thawing should be avoided during culture. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.