Canine Pulmonary Artery Smooth Muscle Cells

Description

Canine Pulmonary Artery Smooth Muscle Cells (CnPASMC) are primary cells isolated from the tunica intima and tunica media layers of healthy pathogen-free canine pulmonary arteries using enzymatic digestion techniques. CnPASMC exhibit a classic spindle-shaped morphology and express smooth muscle-specific markers such as α-smooth muscle actin (α-SMA) and SM22α, verified by immunofluorescence staining. Cells retain functional characteristics of vascular smooth muscle, including contractility and responsiveness to vasoactive stimuli such as endothelin-1 or angiotensin II, making them a robust model for canine pulmonary vascular smooth muscle. Karyotype analysis confirms chromosomal integrity, and donor animals are confirmed free of cardiovascular or systemic disease. To ensure the quality of these primary cells, repeated freeze-thawcycles are not recommended due to the fragile nature of these cells. Each lot undergoes rigorous screening and isolation procedures, and is rigorously tested to ensure it is free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.