Caspase-3 Assay Kit for Live Cells

Description

The Live Cell Assay Kit includes the SuperView 488 Caspase-3 Substrate and the Ac-DEVD-CHO Caspase-3/7 Inhibitor and is designed for sensitive detection of apoptosis based on caspase-3/7 activity. SuperView 488 is suitable for analysis by fluorescence microscopy and flow cytometry. Unlike traditional fluorogenic substrates or FLICA-based fluorescent inhibitors, this substrate detects caspase-3/7 activation in intact cells without inhibiting the apoptotic process. The SuperView 488 substrate consists of a fluorescent DNA-binding dye linked to the caspase-3/7–specific DEVD recognition sequence. In its intact form, the substrate is non-fluorescent and cell-permeable. Upon activation of apoptosis, caspase-3/7 cleaves the DEVD sequence, releasing the high-affinity DNA dye, which translocates to the nucleus, binds DNA, and emits bright green fluorescence. This bifunctional design allows simultaneous detection of caspase activity and visualization of nuclear morphological changes during apoptosis.