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Tumor Cell Lines

CRFK

  • BSL

    1

  • 205
The Cells are free of bovine viral diarrhea(BVD) virus.Tumorigenecity: Isoenzyme: Histopathology: normalSubculture: Remove medium, add fresh 0.25 % Trypsin 0.02 % EDTA solution for 1 to 2 minutes, remove trypsin and allow culture to stand at room temperature ( or at 37'C) until cells detach. Add fresh medium aspirate and dispense into new flasks.Split ratio: […]
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Product Code

CrFK; Crfk; NBL-CrFK; Crandell Reese Feline Kidney; CFK; Crandell Feline Kidney; CCC; Crandell’s Cat Cell

Species

Cat – Female, 12 Week

Cat.No

ABC-TC0194

Product Category Tumor Cell Lines
Size/Quantity

1 vial

Cell Type

Epithelial

Shipping Info

Dry Ice

Growth Conditions

37 ℃, 5% CO2

Source Organ

Kidney

Disease

Normal

Biosafety Level

1

Storage

Liquid Nitrogen

Product Type

Cat Kidney Cell Lines

Description

CRFKCRFK (Crandell-Rees Feline Kidney) is a feline kidney epithelial cell line originally derived from the renal tissue of a healthy adult domestic shorthair cat. CRFK cells exhibit epithelial-like morphology and grow adherently in culture. The stemline karyotype analysis reveals a near-diploid chromosome count of 38 (2n=38). CRFK cells are highly susceptibile to feline calicivirus (FCV) and other feline-specific pathogens. CRFK lacks tumorigenic potential in immunodeficient mice. Due to their permissiveness to viral replication, CRFK cells have become a a cornerstone model for studying feline viral pathogenesis and evaluating antiviral drug compounds.

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Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Application

  • CRFK cells are widely used in viral research and vaccine production. They are particularly valuable for the development of live-attenuated vaccines and biological substances, with a reduced risk of contamination by Endogenous Retroviruses (ERVs). These cells play a pivotal role in investigating the potential relationship between feline vaccines and disease outcomes. Moreover, CRFK cells are extensively used in viral infectivity assays and in studies of retrovirus biology and related vector systems. Their broad utility makes them indispensable for advancing our understanding of various viral infections and their implications for feline health.

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