For research use only
| Cat No. | ABL-TC0172 |
| Product Type | Mouse Sarcoma Cell Lines |
| Species | Mouse |
| Growth Conditions | 37 ℃, 5% CO2 |
| Disease | Sarcoma |
EHS Cell Line from mouse secretes basement membrane matrix proteins including laminin and type IV collagen, serving as a tumor microenvironment model.
The Engelbreth-Holm-Swarm (EHS) cell line is a murine tumor-derived line originating from a spontaneous chondrosarcoma in mice. These cells exhibit an adherent morphology with characteristics typical of epithelial-like tumor cells and are widely recognized for their abundant production of basement membrane (BM) components. Unlike conventional cell lines, EHS cells exhibit a unique characteristic: they cannot be maintained in standard in vitro culture systems and must instead be propagated as tumors in syngeneic mice. EHS cells synthesize high levels of laminin, type IV collagen, entactin, and heparan sulfate proteoglycans, making them essential for generating extracellular matrices that mimic in vivo basement membranes. The cells also express transcription factors including GATA4, GATA6, SOX7, SOX17, CITED1, and CITED2, which regulate differentiation and matrix formation. Additionally, EHS cells contain the necessary post-translational modification machinery—enzymes and chaperones—that support efficient assembly of BM proteins. EHS tumors are tumorigenic in syngeneic mice, forming ECM-rich tumors that closely model the tumor microenvironment. These features make the EHS cell line invaluable for cancer, developmental biology, and cell-matrix interaction research.
| Species | Mouse |
| Cat.No | ABL-TC0172 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Disease | Sarcoma |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Mouse Sarcoma Cell Lines |
The EHS cell line plays a crucial role in producing Matrigel, a reconstituted basement membrane extract abundant in laminin, growth factors, collagen, entactin/nidogen, and heparan sulfate proteoglycan. This extract serves to foster and sustain differentiated phenotypes in cell cultures, finding applications in diverse in vivo contexts. Derived from EHS tumors, these basement membrane extracts effectively mimic the extracellular matrix, thus serving as invaluable substrates for the three-dimensional cultivation of primary tissue-derived human and mouse organoids, iPSC-derived organoids, patient-derived organoids, tumorspheres, human hepatocyte sandwich cultures, as well as in supporting feeder-free cultures of stem cells and neural progenitor cells, and facilitating angiogenesis modeling through the tube formation assay.
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