GLI1 Knockout 293T Cell Line

Description

GLI1 Knockout 293T Cell Line is generated from human embryonic kidney 293T cells through CRISPR/Cas9-mediated disruption of the GLi Family Zinc Finger 1 (GLI1) gene, a terminal effector of the Hedgehog (Hh) signaling pathway that regulates cell proliferation, stemness, and tumorigenesis. This knockout model exhibits impaired Hh pathway activation while maintaining 293T cells’ characteristic adherent growth with epithelial morphology and high transfection efficiency. The cell line serves as a valuable tool for studying developmental biology, cancer stem cell maintenance, and drug resistance mechanisms, particularly in research involving basal cell carcinoma and medulloblastoma. Maintained at low passage numbers (<P20) with genetic stability, the knockout efficiency is validated through genomic PCR, Sanger sequencing, and luciferase reporter assays demonstrating defective Hh signaling. Rigorous quality control confirms the cell line is free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.

Application

• FOR RESEARCH USE ONLY
  

• The GLI1 Knockout 293T Cell Line is a specialized model for studying Hedgehog signaling and its role in development and cancer. This engineered system enables investigation of GLI1’s function as a key transcriptional effector in the pathway. Researchers utilize this knockout line to examine disrupted downstream target gene expression and altered cell proliferation dynamics. The cell line provides a controlled platform for screening Hedgehog pathway inhibitors and developing targeted therapies for basal cell carcinoma and other GLI1-dependent malignancies.