| Species |
Human
|
| Cat.No |
ABC-KH026Y
|
| Quality Control |
All cells test negative for mycoplasma, bacteria, yeast, and fungi.
|
| Product Category |
Transfected Stable Cell Lines |
| Size/Quantity |
1 vial
|
| Cell Type |
Epithelial
|
| Shipping Info |
Dry Ice
|
| Growth Conditions |
37 ℃, 5% CO2
|
| Source Organ |
Kidney
|
| Disease |
Normal
|
| Biosafety Level |
1
|
| Storage |
Liquid Nitrogen
|
| Product Type |
Knockout Stable Cell Line
|
| Host Cell |
293T
|
| Gene Info |
GLI1
|
Description
GLI1 Knockout 293T Cell Line is generated from human embryonic kidney 293T cells through CRISPR/Cas9-mediated disruption of the GLi Family Zinc Finger 1 (GLI1) gene, a terminal effector of the Hedgehog (Hh) signaling pathway that regulates cell proliferation, stemness, and tumorigenesis. This knockout model exhibits impaired Hh pathway activation while maintaining 293T cells’ characteristic adherent growth with epithelial morphology and high transfection efficiency. The cell line serves as a valuable tool for studying developmental biology, cancer stem cell maintenance, and drug resistance mechanisms, particularly in research involving basal cell carcinoma and medulloblastoma. Maintained at low passage numbers (<P20) with genetic stability, the knockout efficiency is validated through genomic PCR, Sanger sequencing, and luciferase reporter assays demonstrating defective Hh signaling. Rigorous quality control confirms the cell line is free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.
