GSDMD Knockout THP-1 Cell Line

Description

GSDMD Knockout THP-1 Cell Line is generated from human monocytic THP-1 cells through CRISPR/Cas9-mediated disruption of the Gasdermin D (GSDMD) gene, a key executor of pyroptosis that mediates inflammatory cell death through membrane pore formation. This knockout model exhibits defective pyroptotic cell death while maintaining typical THP-1 characteristics including suspension growth with round morphology and PMA-induced differentiation into macrophage-like cells. The cell line provides an essential tool for studying inflammasome signaling, inflammatory responses, and host-pathogen interactions, particularly in research involving NLRP3 inflammasome activation and IL-1β/IL-18 secretion. Maintained at low passage numbers (<P20) with stable genotype, the knockout efficiency is validated through genomic PCR, sequencing, and functional assays demonstrating impaired pyroptosis. The cell line has been rigorously tested and confirmed free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.