HDAC4 Knockout 293T Cell Line

Description

HDAC4 Knockout 293T Cell Line is generated from human embryonic kidney 293T cells through CRISPR/Cas9-mediated disruption of the Histone Deacetylase 4 (HDAC4) gene, a class IIa histone deacetylase that regulates gene expression through chromatin remodeling and plays critical roles in muscle differentiation, neuronal survival, and bone development. This knockout model exhibits altered epigenetic regulation and transcriptional activity while maintaining 293T cells’ characteristic adherent growth with epithelial morphology and high transfection efficiency. The cell line serves as an essential tool for studying epigenetic mechanisms, cellular differentiation, and neurodegenerative diseases, particularly in research involving MEF2 signaling pathways and calcium-dependent gene regulation. Maintained at low passage numbers (<P20) with genetic stability, the knockout efficiency is validated through genomic PCR, Sanger sequencing, and Western blot analysis. Rigorous quality control confirms the cell line is free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.