Human Acute Lymphoid Leukemia Peripheral Blood Mononuclear Cells (Newly Diagnosed/Untreated) are primary mononuclear cells isolated from the peripheral blood of patients diagnosed with Acute Lymphoid Leukemia (ALL). Collected via gradient centrifugation and PBS dilution, the population includes lymphocytes (T cells, B cells, NK cells) and monocytes, key for hematologic immune responses. These cells exhibit apoptosis resistance and distinct gene expression, including increased caspases-8/9 and pro-apoptotic protein BAK compared to healthy PBMCs. Typically grown in suspension culture (37°C, 5% CO₂), these cells can also be sorted for further studies on leukemia pathogenesis, immune interactions, and therapeutic screening. They are tested negative for mycoplasma, bacteria, yeast, and fungi, cryopreserved for optimal viability. Ideal for immunophenotyping, apoptosis analysis, and drug sensitivity testing in ALL.
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