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Human AKT1 (D323H_C310S) BAF3 Cell Line

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Home | Transfected Stable Cell Lines | Overexpression Stable Cell Lines | Human AKT1 (D323H_C310S) BAF3 Cell Line
Species

Human
Cat.No

ABC-X0030C
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Product Category Transfected Stable Cell Lines
Size/Quantity

1 vial
Cell Type

Lymphocyte
Shipping Info

Dry Ice
Growth Conditions

37 °C, 5% CO₂
Source Organ

Lymphatic
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Overexpression Stable Cell Lines
Host Cell

BAF3

Description

Human AKT1 (D323H_C310S) BAF3 Cell Line is a genetically engineered model derived from selected murine Ba/F3 parental cell line based on customers’ requirement. AKT1 (D323H_C310S) BAF3 mutant cell line is generated by stable integration of exogenous human AKT1 gene harboring dual mutations (D323H and C310S) into Ba/F3 host cells using our optimized transduction of lentiviral vectors.
Target
AKT1 is a central serine/threonine kinase involved in cell survival, metabolism, and proliferation via the PI3K/AKT/mTOR pathway. The C310S and D323H mutations are located in the kinase domain and have been associated with altered kinase activity and tumorigenesis. These mutations facilitate the study of structure-function relationships and resistance mechanisms to AKT inhibitors. AcceGen offers generation of stable overexpression and dual-mutant cell lines targeting any gene of your interest. Polyclonal or monoclonal is optional based on customers’ research needs.

AcceGen Frozen Cells & Cell Lines 1 vial
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Application

  • For research use only

  • The AKT1 (D323H_C310S) BAF3 Cell Line serves as a powerful tool to investigate altered AKT signaling and therapeutic response. This model enables mechanistic studies of dual mutation effects, IL-3-independent proliferation, and sensitivity to PI3K/AKT pathway inhibitors. It is utilized for oncology drug screening and personalized therapy research.

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